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CCD-112CoN
CCD-112CoN
規(guī)格:
貨期:
編號(hào):B164178
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CCD-112CoN
商品貨號(hào) B164178
Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 22 weeks gestation
Gender female
Karyotype 46, XX; modal number = 46; polyploidy at 5.5%; inv(9)(p13q13)
Clinical Data
female
Comments
The line begins to senesce at population doubling level (PDL) = 76.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,13
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,9
THO1: 6,7
TPOX: 8
vWA: 16,18
Population Doubling Capacity The line begins to senesce at population doubling level (PDL) = 76.
Name of Depositor ATCC
Deposited As Homo sapiens
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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