| 產(chǎn)品名稱 |
IgG-11H4 |
| 商品貨號(hào) |
B164836 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
Immunoglobulin; monoclonal antibody; against the carboxy terminus of lipoprotein receptor related protein (LRP) |
| Cellular Products |
Immunoglobulin; monoclonal antibody; against the carboxy terminus of lipoprotein receptor related protein (LRP) |
| Comments |
Animals were immunized with a synthetic peptide (corresponding to the 13 carboxy terminal peptides of human LRP) coupled to KLH.
The antibody reacts with the carboxy terminus of LRP from various species. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: 1 to 2 times per week |
| Cryopreservation |
Complete growth medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
YK Ho, JL Goldstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kowal RC, et al. Low density lipoprotein receptor-related protein mediates uptake of cholesteryl esters derived from apoprotein E-enriched lipoproteins. Proc. Natl. Acad. Sci. USA 86: 5810-5814, 1989. PubMed: 2762297
Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029
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