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A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.

The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.

LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages.

The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cell density between 1 x 10⁵ and 1 x 10⁶ viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). 

Generation of conditioned Medium
LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420).  LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.RefOlivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

1. Allow cells to become confluent.
2. After 5 to 7 days, collect supernatant, centrifuge at 125 x g for 5 to 10 minutes 
3. Filter (200 nM filter)
4. Store aliquots at –20°C.

Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247