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Animals were immunized with purified nuclei from Xenopus laevis oocytes.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with a conserved phosphorylated epitope on SR proteins.

SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with masses of 75000, 70000, 55000, 40000, 30000 and 20000 daltons.

Zahler AM, et al. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6: 837-847, 1992. PubMed: 1577277

Roth MB, et al. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115: 587-596, 1991. PubMed: 1717489

Zahler AM, et al. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260: 219-222, 1993. PubMed: 8385799

Roth MB, et al. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle. J. Cell Biol. 111: 2217-2223, 1990. PubMed: 1703534

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.