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MOLT-4
MOLT-4
規(guī)格:
貨期:
編號(hào):B165184
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MOLT-4
商品貨號(hào) B165184
Organism Homo sapiens, human
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute lymphoblastic leukemia
Age 19 years
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17.
Images
Derivation
MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552). The line was established from cells taken from a patient in relapse.
Clinical Data
male
19 years
Antigen Expression
CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)
Genes Expressed
high levels of terminal deoxynucleotidyl transferase (TdT) are produced.
Cellular Products
high levels of terminal deoxynucleotidyl transferase (TdT) are produced
Tumorigenic Yes
Effects
Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice
Comments
The patient had received prior multidrug chemotherapy.
There is a G -> A mutation at codon 248 of the p53 gene.
P53 is not expressed.
The cells do not produce immunoglobulin or Epstein-Barr virus.
The line was originally contaminated with mycoplasma but has been cured with antibiotics.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 105 cells/mL and subculture before the cell density reaches 2 X 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: XY
CSF1PO: 11, 12, 13
D13S317: 12, 13
D16S539: 11, 14
D5S818: 12
D7S820: 8, 10, 11
THO1: 6, 8
TPOX: 8
vWA: 17, 18
Name of Depositor J Minowada
Deposited As Homo sapiens
References

Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523

Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323

Cross References

Nucleotide (GenBank) : AF146433 Homo sapiens cell-line MOLT-4 sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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