国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

The clone N1E was subcloned by isolation of single cells on glass shards.

The adrenergic clone N1E-115 exhibits high levels of activity of the enzymes tyrosine hydroxylase and acetylcholinesterase which are necessary for neurotransmitter synthesis but are almost devoid of choline acetyltransferase.

These cells contain 14 receptors for neurotransmitters.

1. Remove medium, rinse with Modified Puck's Saline D1 solution:
   1. Glucose                       5.5 mM
   2. KCl                             5.4 mM
   3. Sucrose                     58.4 mM
   4. Na₂HPO₄-7H₂O         0.17 mM
   5. NaCl                          138 mM
   6. KH₂PO₄                     0.22 mM
   Adjusted to a pH of 7.4±0.05 with 0.1 N NaOH or 0.N HCl Adjusted to an osmomolality of 340±5 mosM with either sucrose or H₂0 to decrease or increase the osmomolality.
2. Allow flasks to remain at room temperature until cells detach.
3. To remove saline, add fresh culture medium, transfer cell suspension to centrifuge tube and spin at approximately 125 x for 5 to 10 minutes.
4. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
5. Place culture vessels in incubators at 37°C

Note: If DMEM formulation contain 1.5 g/L sodium bicarbonate.

. Methods in neurotransmitter receptor analysis. New York: Raven Press; 1990.

Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933

Amano T, et al. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase- acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc. Natl. Acad. Sci. USA 69: 258-263, 1972. PubMed: 4400294

Richelson E. Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone N1E-115. J. Neurochem. 21: 1139-1145, 1973. PubMed: 4148612