国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > NCTC clone 1469 [derivative of NCTC 721]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
NCTC clone 1469 [derivative of NCTC 721]
NCTC clone 1469 [derivative of NCTC 721]
規(guī)格:
貨期:
編號:B165383
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 NCTC clone 1469 [derivative of NCTC 721]
商品貨號 B165383
Organism Mus musculus, mouse
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age newborn
Gender male
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 41; range = 38 to 86.
Two marker chromosomes present: One metacentric with one pair of arms having achromatic gaps near centromere; and an acrocentric with satellites.
Antigen Expression
H-2k
Virus Susceptibility Vesicular stomatitis virus
Human poliovirus 1
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing Subcultures are prepared by scraping. Remove old medium, add fresh medium and dislodge the cells from the floor of the flask. Aspirate cells and dispense the suspension into new flasks. A standard trypsinizaton may be used if desired. The culture medium in heavy cultures may be turbid in appearance and and give the gross impression of bacterial contamination. It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid. The shed cells are viable and may be used to initiate new cultures.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor VJ Evans
Deposited As Mus musculus
Year of Origin April, 1952
References

Hobbs GL, et al. Establishment of a clone of mouse liver cells from a single isolated cell. J. Natl. Cancer Inst. 18: 701-707, 1957. PubMed: 13502690

Evans VJ, et al. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst. 12: 1245-1265, 1952. PubMed: 14939026

J. Natl. Cancer Inst. 23: 823, 1959.

J. Natl. Cancer Inst. 27: 29, 1961.

Evans VJ, et al. Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. Cancer Res. 12: 261-266, 1958. PubMed: 13523589

. . Fed. Proc. 17: 967, 1958.

Westfall BB. Characterization of cells in tissue culture. Natl. Cancer Inst. Monogr. 7: 147-158, 1962. PubMed: 14006338

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
陇西县| 阿拉尔市| 凤山县| 胶州市| 扎赉特旗| 德令哈市| 桦南县| 宁都县| 明水县| 灵武市| 车致| 潞城市| 容城县| 甘洛县| 西昌市| 红桥区| 双鸭山市| 灯塔市| 龙游县| 沈阳市| 定安县| 洛扎县| 兴和县| 吐鲁番市| 子洲县| 微山县| 白银市| 南宁市| 岱山县| 青龙| 无棣县| 南岸区| 枣庄市| 麻栗坡县| 襄城县| 孙吴县| 兴宁市| 高青县| 阿勒泰市| 长乐市| 仲巴县|