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Pfeiffer
Pfeiffer
規(guī)格:
貨期:
編號(hào):B165496
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Pfeiffer
商品貨號(hào) B165496
Organism Homo sapiens, human
Tissue derived from metastatic site: pleural effusion
Cell Type lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Age adult
Gender male
Ethnicity White
Applications
This cell line is a model system for studying non-Hodgkin lymphomas.
Karyotype The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
Derivation
The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.

Clinical Data
male
White
adult
Antigen Expression
CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 -
Comments
The cells are negative for Epstein-Barr virus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 - 5 x 105 viable cells/mL. Maintain cell density between 3 x 105 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Isotype IgG kappa
Population Doubling Time 24 to 30 hrs
Name of Depositor H Ben-Bassat
Deposited As human
Year of Origin 1992
References

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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