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Animals were immunized with the Peyer's patch HEV binding lymphoma line TK1.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyer's patch.

This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical).

Holzmann B, et al. Identification of a murine Peyer's patch-specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4alpha. Cell 56: 37-46, 1989. PubMed: 2463092

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.