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Size (kb): 6.8280000686645510

Vector: pMS-TRP (phagemid)

Promoters: Promoter GAL

Marker(s):TRP1,ampR

Construct size (kb): 6.828000068664551

Features: marker(s): TRP1
marker(s): ampR
other: Myristoylation site
other: lox site
promoter: GAL
promoter: T7
replicon: 2 micron ori
replicon: f1
replicon: pMB1
MCS: EcoRI...SphI
restriction site: NotI
terminator: CYC1
epitope tag: 3x hemaglutinin (HA)

vector component of two-hybrid screening system

Restriction digests of the clone give the following sizes (kb): HindIII--4.0, 2.8; BglI/XhoI--4.5, 2.3; SphI--6.8.

SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein.

Vector for use the the SOS Recruitment System (SRS). It encodes the v-Src myristoylation signal and can be used to construct expression libraries to be screened for interacting proteins.

Temperature: 37.0°C