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Temperature: 37°C

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 0.001). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere. End adsorption by adding virus growth medium.

Incubation: 2 to 6 days

Zheng ZM, et al. First isolation of enterovirus type 71 from vesicle fluid of an adult patient with hand-foot-mouth disease in China. Virologica Sinica. 4: 375-382, 1989.

Zheng ZM, et al. Comparison of enterovirus 71 (E71) isolated from a patient with hand-foot-and-mouth disease in China to prototype E71 BrCr strain by polymerase chain reaction using a unique primer pair. Clin. Diagn. Virol. 1: 137-139, 1993. PubMed: 15566727

Zheng ZM, et al. Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5'-noncoding region. J. Med. Virol. 47: 161-167, 1995. PubMed: 8830120