国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pNK2859
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pNK2859
pNK2859
規(guī)格:
貨期:
編號:B222996
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pNK2859
商品貨號 B222996
Designations pNK2859
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors N Kleckner
Applications
Produces protein transposase, Tn10, ats1 ats2 (ATS)
Vector
Construct size (kb): 6.5
Insert
DNA: Genomic
Insert lengths (kb): 4.40
Gene product: transposase, Tn10, ats1 ats2 (ATS)
Target Gene: transposase, Tn10, ats1 ats2 (ATS)
Insert Size (kb) 4.40
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 2.1; HindIII--4.6, 1.2, 0.78; PstI--6.6.

This cassette is inserted into the HindIII site (original nt 2272) of the ATS transposase-containing EcoRI fragment of pNK2881, ATCC 77351.

Construct useful for general transposon mutagenesis in Escherichia coli.

pBR322 was modified by deletion of bp 75-2352 and destruction (by filling in) of the HindIII site.

Contains the ats1 ats2 transposase gene that permits relaxed insertion specificity (altered target specificity, ATS). Expression is regulated by the Ptac promoter, inducible by IPTG.

The transposase segment extends from IS10R to the EcoRI site at nt 3140 of Tn10, with a deletion of nucleotides 1329-1942 to remove the transposase binding site.

Contains a 1.8 kb mini-Tn10 cassette conferring kanamycin resistance, bounded by inverted repeats of the outermost 70 bp of IS10R and embedded in 40 bp of lambdacI terminating in HindIII sites.

Transcription from the Ptac promoter is in a direction opposite to that of amp.

The order of the major features in this plasmid is: EcoRI - Ptac - ATS transposase - mini-Tn10 kan - EcoRI - pMB1 ori - ampR.

Classification Enterobacteriaceae, Escherichia
References

Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
平遥县| 治多县| 监利县| 曲阜市| 建昌县| 芜湖县| 二连浩特市| 大洼县| 夏邑县| 友谊县| 封丘县| 盐津县| 孝感市| 临朐县| 措勤县| 佛坪县| 铜梁县| 丹巴县| 建瓯市| 沿河| 常德市| 平阴县| 桐乡市| 遂溪县| 杭锦后旗| 正安县| 阿尔山市| 正阳县| 大竹县| 南和县| 夹江县| 眉山市| 琼结县| 呼伦贝尔市| 莒南县| 民权县| 民丰县| 呼伦贝尔市| 澜沧| 大庆市| 宜兰县|