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產(chǎn)品名稱(chēng)	pYAC4
商品貨號(hào)	B223672
Designations	pYAC4
Depositors	MV Olson
U.S. Patent	4,889,806
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level	1
Vector Information	Size (kb): 11.4540004730224600 Vector: pYAC4 (YAC) Marker(s): TRP1,URA3,ampR,HIS3,SUP4 Construct size (kb): 11.454 Features: marker(s): HIS3 marker(s): SUP4 marker(s): TRP1 marker(s): URA3 marker(s): ampR other: telomere replicon: ARS1 replicon: pMB1 centromere: CEN4
Applications	YL-type (YAC) shuttle vector Shuttle vector Vector for constructing genomic libraries
Comments	Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37°C
References	Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825 Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741 Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791 Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163
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