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Construct size (kb): 34.8

DNA: genomic

Insert lengths(kb): 5.80

Gene product: tubulin, beta [tubb]

Restriction digests of the clone give the following sizes (kb): SalI/BglII--20.0, 9.4, 7.7, 5.8, 1.3; BamHI--21.5, 12.2, 10.5; BglII-->23.0, 9.4, 7.7, 1.3; EcoRI--21.5, 12.2, 4.2, 3.25, 2.4.

Cloned from a rat isolate.

The sequenced portion predicts the following restriction sites (approximate kb from a 5' HindIII site 3 kb from the SalI end of the SalI/BglII fragment): BamHI--1.8; HindIII--1.45, 1.76; EcoRI--2.2, 2.4; KpnI--1.9; SacI--2.3; PvuI--1.26.

A 1.1 kb HindIII/BglII fragment detects genomic restriction fragments of the following sizes (kb): BglII--5.8; HindIII--2.6; BglII/HindIII--1.1.

Derived from a single copy gene that occurs on a chromosome of 485 kb.

The complete gene is contained in a 5.8 kb BglII fragment of this clone (excised by a BglII/SalI digest). The GenBank record was derived from 2876 bp of that fragment.