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Size (kb): 5.5000000000000000

Vector: pUMSV0CAT (plasmid)

Promoters: Promoter none

Construction: pSV0cat, UMS of c-mos

Marker(s):ampR,cmlS

Construct size (kb): 5.5

Features: insert detection: CAT
marker(s): ampR, cmlS
promoter: none
replicon: pMB1

promoter-cloning vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7.

The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells.

A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene.

A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi.

Temperature: 37.0°C