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Size (kb): 7.1640000343322750

Vector: pCMVbeta (plasmid)

Promoters: Promoter CMV immediate early

Construction: pUC19, pC4AUG beta-gal, pCM5029, pL1

Marker(s):ampR

Construct size (kb): 7.164000034332275

Features: eukaryotic promoter/expression cassette: CMV promoter/enhancer SV40 splice signal
eukaryotic reporter gene cassette: lacZ
marker(s): ampR
promoter: CMV immediate early
replicon: pMB1
reporter group: lacZ
terminator: SV40 late
enhancer: CMV immediate early

contains easily purifiable cassette(s) for construction

expression vector

reporter construct

vector permitting visual detection of activity in colonies

Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2; NotI/EcoRI--3.54, 2.9, 0.9.

Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.

Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.

The lacZ gene may be excised by NotI and replaced with alternative sequences.

The order of the major features in this plasmid is: pUC19 - EcoRI - PstI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - PstI - HindIII - pUC19.

Temperature: 37.0°C