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Size (kb): 5.5000000000000000

Vector: pUR18N (plasmid)

Promoters: Promoter lac

Construction: pUR18 (ATCC 77297)

Marker(s):ampR,ura4+

Construct size (kb): 5.5

Features: insert detection: lacZ'
marker(s): ampR, ura4
promoter: lac
replicon: pMB1, ars1

expression vector

shuttle vector

vector for other uses

vector permitting construction of fusion proteins

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5.

Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation.

The addition of NotI and SfiI sites to the MCS enables unidirectional exonuclease III deletions and facilitates the rescue in E. coli of plasmids where dimerization has occurred.

The order of the major features in this plasmid is: ars1 - ura4 - SfiI/MCS/EcoRI/lacZ' - pMB1 ori - ampR.

Temperature: 37.0°C