国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

Size (kb): 4.320

Vector: pFN476 (plasmid)

Promoters: Promoter lac

Construction: pGEM3Z, pKA101-6

Marker(s):ampR

Construct size (kb): 4.320

Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR

expression vector T7 promoter

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

vector with low copy number

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.

Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.

Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.

Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.

T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).

Temperature: 37.0°C