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lymphoblast cell line GM03714 (46, XX)

Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.

Tissue: lymphoblast cell line GM03714 (46, XX)

Chromosome: 2q21-q22

DNA: genomic

Digest: Complete

Insert size range: 50-600 bp

Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.

Approximately 50% of the clones contain repetitive sequences. Of 37 single copy clones tested, 26 (70%) hybridized to chromosome 2.

Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.