国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pKSM718
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pKSM718
pKSM718
規(guī)格:
貨期:
編號(hào):B227149
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pKSM718
商品貨號(hào) B227149
Designations pKSM718
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Vector Information
Size (kb): 3.5840001106262210
Vector: pKSM718 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC18
Marker(s):ampR
Construct size (kb): 3.584000110626221
Features: insert detection: lacZalpha
marker(s): ampR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: DsaI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
Applications
expression vector
vector permitting RNA synthesis in vitro
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; BglI--3.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglI--3.29; EcoRI--0.39; NcoI--0.33; PvuI--0.51, 3.04; PvuII--0.48.
Differs from pKSM717 (ATCC 87162) only in the orientation of the ampicillin resistance gene.
Expression vector allowing generation of N-terminal deletions or modifications of foreign DNA cloned into the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
广饶县| 宜良县| 磐安县| 保亭| 临汾市| 大荔县| 剑川县| 沧州市| 库车县| 普定县| 赣州市| 阿拉善左旗| 稷山县| 邻水| 福清市| 监利县| 南充市| 馆陶县| 临沂市| 龙海市| 耒阳市| 宝丰县| 明水县| 银川市| 海伦市| 绵阳市| 根河市| 五莲县| 台安县| 东方市| 宝坻区| 城固县| 大安市| 秭归县| 惠东县| 黎川县| 郎溪县| 博客| 崇明县| 家居| 常山县|