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pmetH [p met H]
pmetH [p met H]
規(guī)格:
貨期:
編號:B233127
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pmetH [p met H]
商品貨號 B233127
Designations pmetH [p met H]
Species Homo sapiens, human
Depositors GF Van de Woude
Applications
DNA from secondary transfectants was used to isolate the clone.
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.600000023841858
Tissue: HOS cell line DNA transferred into NIH 3T3 cells
Gene product: met proto-oncogene(hepatocyte growth factor receptor) [MET]
Alleles: D1, D2, D1, D2, A2, B2, A2, B1, B2, A1, A1, A2, A2, B1, C1, C2, A1, A1, A1, A2, A2, C1, C2, A1
Insert Size (kb) 1.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI/EcoRI--4.2, 1.25; SalI--5.5; EcoRI--5.5.
NIH 3T3 cells were transformed with DNA from the osteosarcoma HOS cell line which had been treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). DNA from secondary transfectants was used to isolate the clone.
References

Park M, et al. Sequence of MET protooncogene cDNA has features characteristic of the tyrosine kinase family of growth factor receptors. Proc. Natl. Acad. Sci. USA 84: 6379-6383, 1987. PubMed: 2819873

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

White R, et al. A closely linked genetic marker for cystic fibrosis. Nature 318: 382-384, 1985. PubMed: 3906407

Cooper CS, et al. Molecular cloning of a new transforming gene from a chemically transformed human cell line. Nature 311: 29-33, 1984. PubMed: 6590967

Dean M, et al. The human met oncogene is related to the tyrosine kinase oncogenes. Nature 318: 385-388, 1985. PubMed: 4069211

Lathrop GM, et al. Refined linkage map of chromosome 7 in the region of the cystic fibrosis gene. Am. J. Hum. Genet. 42: 38-44, 1988. PubMed: 2892400

Julier C, White R. Detection of a NotI polymorphism with the pmetH probe by pulsed-field gel electrophoresis. Am. J. Hum. Genet. 42: 45-48, 1988. PubMed: 2892401

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