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Leishmania tarentolae Parrot
Leishmania tarentolae Parrot
規(guī)格:
貨期:
編號:B233265
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Leishmania tarentolae Parrot
商品貨號 B233265
Strain Designations Tar II
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
lizard, Tarentola mauritanica, Biakra, Algeria, 1934
Product Format frozen
Type Strain no
Comments
Mutant of W. Trager strain (ATCC 30267). Promastigotes.
Effect of temperature on growth and nutritional requirements
lipids
Heterogeneity of minicircles
isoenzyme electrophoresis for species identification
Variation in kinetoplast minicircle and maxicircle DNA sequences
Cyclopropane fatty acid
Medium ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Cryopreservation

1.?? Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.

2. ? Adjust concentration of cells to 2 x 107/ml in fresh medium.

3. ?  While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in Locke?s solution.? The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.

4.   Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.

5. ? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.?? Place the ampules in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately -1°C/min.) ?

7.?? Store in either the vapor or liquid phase of a nitrogen refrigerator.

8.?? To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.? Do not agitate the ampule.? Do not leave ampule in water bath after thawed.

9.?? Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 1011.?

10.????????? Incubate vertically at 25°C with the cap screwed on tightly.

11.????????? Maintain as described above.?

Name of Depositor SM Krassner
Chain of Custody
ATCC <<--SM Krassner<<--W. Trager <<--- L. Parrot
Year of Origin 1934
References

Krassner SM. Effect of temperature on growth and nutritional requirements of Leishmania tarentolae in a defined medium. J. Protozool. 12: 73-78, 1965. PubMed: 14292557

Beach DH, et al. Lipids of Leishmania promastigotes. J. Parasitol. 65: 201-216, 1979. PubMed: 448607

Englund PT, et al. A nicked form of kinetoplast DNA in Leishmania tarentolae. J. Biol. Chem. 252: 6208-6216, 1977. PubMed: 893402

Challberg SS, Englund PT. Heterogeneity of minicircles in kinetoplast DNA of Leishmania tarentolae. J. Mol. Biol. 138: 447-472, 1980. PubMed: 6251227

Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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