国产精品国产精品一区精品国产自在现偷99精品国产在热2019国产拍偷精品网国产精品视频全国免费观看,国产精品v欧美精品v日韩精品青青精品视频国产久久国产精品久久精品国产亚洲精品国产精品国产欧美精品一区二区三区,国产精品第一页国产亚洲精品国产福利国产精品自拍国产精品视频在线观看亚洲国产精品一区二区久久国产精品国产三级国产专不,国产精品视频大陆精大陆国产国语精品2019精品国产品对白在线285年香蕉精品国产高清自在自线隔壁老王国产在线精品在线观看精品国产福利片,国产三级精品三级在专区精品国产自在现偷国产精品一区二区三区国产日韩精品欧美一区喷水亚洲精品国产精品国自产国产在线精品一区二区不卡

tumor model

The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. MiF-6 (ATCC CRL-2802) and M-7 (ATCC CRL-2804) are derivatives of MC-TGS17-51 (ATCC CRL-2799). M7 cells were constructed by transfection of MC-TGS17-51 cells with the pCR3.1 vector alone. The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene. M7 cells are used as a control for MiF-6. MiF-6 cells were constructed by transfection of MC-TGS17-51 cells with pRNAi-5 ligated to pCR3.1 to produce a construct expressing mouse double stranded short-interfering ribonucleic acid (siRNA) formaldehyde dehydrogenase (FDH) mRNA. The pRNAi-5 construct targeted the sequence from -18 to +4 of the mRNA, including the start codon. M7 and MiF-6 cells are 6-thioguanine sensitive, neomycin (G418) resistant, hypoxanthine phosphoribosyl transferase positive (HPRT+) and will grow in HAT selection medium. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.

male

mouse siRNA formaldehyde dehydrogenase (FDH)

mouse siRNA formaldehyde dehydrogenase (FDH)

Yes, forms subcutaneous tumors in syngeneic C57BL/6 mice.

The Mutatect mouse tumor model is a series of cell lines (eight are available from ATCC) derived from a murine fibrosarcoma that can grow in culture as well as form subcutaneous tumors in syngeneic C57BL/6 mice. MiF-6 (ATCC CRL-2802) and M-7 (ATCC CRL-2804) are derivatives of MC-TGS17-51 (ATCC CRL-2799). M7 cells were constructed by transfection of MC-TGS17-51 cells with the pCR3.1 vector alone. The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene. M7 cells are used as a control for MiF-6. MiF-6 cells were constructed by transfection of MC-TGS17-51 cells with pRNAi-5 ligated to pCR3.1 to produce a construct expressing mouse double stranded short-interfering ribonucleic acid (siRNA) formaldehyde dehydrogenase (FDH) mRNA. The pRNAi-5 construct targeted the sequence from -18 to +4 of the mRNA, including the start codon. M7 and MiF-6 cells are 6-thioguanine sensitive, neomycin (G418) resistant, hypoxanthine phosphoribosyl transferase positive (HPRT+) and will grow in HAT selection medium. The Mutatect cell lines can be used in vitro/in vivo for the detection of deletion mutations of the hypoxanthine phosphoribosyl transferase (HPRT) gene. They are also useful for the study of inflammatory infiltration of solid tumors.

Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
   An inoculum of 2 X 10(3) to 6 X 10(3) viable cells/cm2 is recommended.
6. Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 5 X 10(3) and 3 X 10(5) cells/cm2

Subcultivation Ratio: A subcultivation of 1:4 to 1:6 is recommended

Medium Renewal: Two to three times weekly

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C